Understanding HPLC-UV and Mass Spectrometry

High-Performance Liquid Chromatography (HPLC) coupled with Ultraviolet (UV) detection and Mass Spectrometry (MS) forms the backbone of modern peptide analysis.

Why 99% Purity Matters

In analytical terms, purity refers to the percentage of the target peptide sequence relative to all peptide-related impurities. Impurities often include truncation sequences (missing amino acids) or deletion sequences.